Home > News and events > Newsletters > Skip DNA extraction / Plant NA extraction / PCR cleanup / Real filter tips

Skip DNA extraction / Plant NA extraction / PCR cleanup / Real filter tips

Be more efficient. PCR without DNA extraction

Extraction of DNA is a time-consuming step to get to your final goal. With Versataq it is possible to skip your DNA extraction and go direct to PCR using any type of crude sample.

VersaTaq is a mutant Taq DNA polymerase for Direct PCR from your sample. It is resistant to common PCR inhibitors, allowing you to simply forget about the need for costly and time consuming extraction and purification processes. Go Direct to PCR!

VersaTaq polymerase works perfectly in a wide range of PCR applications:

  • Whole blood (up to 40%)
  • Feces
  • Blood spots on sample collection cards
  • Animal Tissue
  • Plant Tissue
  • Buccal swabs
  • Soil
  • Urine
  • Crude extracts

Interested in knowing more? click here for the product sheet

Would you like to test it and see how easy it is? click here to contact our sales support.

Does your filter tip suck at aerosols?

Filter tips mounted in air-displacement pipettes are used to prevent potential contamination of the pipette cones by e.g. DNA fragments.

The more effective the filter, the lower the risk of contamination. This is of utmost importance for preventing false-positive results, especially in highly sensitive PCR experiments.

Double-stranded DNA, the main product of PCR, is very long lived. It therefore seems obvious that pipetting of DNA or PCR product is one of the most insecure steps in the PCR procedure. Cross-contamination occurs if incorrect pipetting causes splashes or drips. Yet even if pipettes are handled properly, aerosols can still be generated, containing DNA molecules which can thus contaminate the pipette and then subsequent different batches. This demands increasing efforts to reduce penetration of contaminants by means of filter tips. Filter tips mounted in air-displacement pipettes are used to prevent potential contamination of the pipette cones by e.g. DNA fragments. The more effective the filter, the less the risk of contamination. This is of utmost importance for preventing false-positive results, especially in highly sensitive PCR experiments. The filter in all TipOne® filter tips is made of ultra-pure, high-density polyethylene that protects against aerosol cross-contamination without trapping your valuable samples. Starlab TipOne 200 μl filter tips have been compared with those of nine other manufacturers.

Read the full report here

Having issues isolating DNA and RNA from plant material?

The extraction of nucleic acids from plant material is often hindered by the presence of secondary metabolites, such as polyphenolic compounds, tannins or polysaccharides, which influences the sample quality. This can affect your downstream applications.
Isogen Life Science offers different plant kits for DNA and RNA isolation, using spin columns and magnetic beads. Over the years we have received a large amount of positive feedback from satisfied users of our kits, active in the Plant- and Seed breeding companies. Two kits are highlighted below:

RNA: InviTrap® Spin Plant RNA Mini Kit

To obtain high quality RNA, efficient and complete disruption and homogenization of the plant sample is essential.

The Invitrap total RNA isolation kit combines efficient lysis and homogenization of the starting material in the presence of a highly denaturing buffer to inactivate RNAses and efficiently remove DNA. The lysis solution avoids binding of inhibiting secondary metabolites to the DNA-binding carrier particles, avoiding competitive binding of DNA.

Possible downstream applications for which the highly purified total RNA sample can be used:

  • Northern Blotting
  • RNA dot blots
  • cDNA-library
  • in vitro translation
  • RT-PCR*
  • DDRT-PCR*
  • TaqMan® analysis and array technologies.

Interested? download the product information here.

DNA: InviMag® Plant DNA Mini Kit/ KFmL

To obtain high yields of DNA, the complete and quick disruption of the plant sample is essential. The amount of purified DNA obtained from the plant material depends on sample source, transport conditions, storage and age of the sample.

Using the InviMag® Plant DNA Mini Kit/ KFmL procedures, the plant material is first mechanically disrupted and then lysed by addition of lysis buffer and Proteinase K. After lysis nondissolved structure proteins, polysaccharides and other cell debris are precipitated in a single brief centrifuge step. After clearing the DNA binds to the magnetic beads whereas inhibitors are efficiently removed during the following wash steps and highly purified DNA is eluted customer defined volume of elution buffer.

This procedure requires minimal interaction by the user, allowing safe handling of samples and are designed to avoid sample-to-sample cross contamination.

Downstream applications for which the highly purified DNA sample can be used:

  • PCR*
  • Genotyping
  • Restriction digestion

Would you like to know more? Click here for the product brochure.

The world’s fastest PCR cleanup

Isogen Life Science offers PCR cleanup kits, which are designed for a fast and efficient removal of impurities from your DNA samples. how fast can you go?

MSB® Spin PCRapace

Highly concentrated, pure DNA fragments and PCR products are isolated from the solution by using a binding elution. Therefore a washing step is not required.

The purification procedure has been optimized to remove salts, enzymes, nucleotides, mineral oil, agarose, ethidium bromide and other impurities from DNA samples.

Advantages:

  • convenient and fast sample processing
  • the most efficient removal of contaminants
  • high recovery rate of PCR products or DNA fragments
  • broad range of fragment sizes 80 bp - 30 kb can be purified

Interested? Click here for the product sheet.

Are you paying too much for your cDNA?

High Performance, High Value: VeriScript Reverse Transcriptase!

Engineered version of M-MLV RT for Reverse Transcriptase applications and the highest cDNA yields.

  • Active over a wide temperature range. Recommended incubation temperature of 42°C, but incubation temperatures up to 50°C may be used for RNA templates with difficult regions containing secondary structures or GC-rich regions.
  • Compatible with oligo dT, random; or gene-specific priming.
  • VeriScript costs as little as 1.2 ¢ per unit

Applications:

  • RT-PCR, qRT-PCR
  • Synthesis of first strand cDNA
  • Amplification of RNA
  • Primer extension assays
  • cDNA cloning
  • Library construction for RNA-seq

Combine VeriScript with our RNase Inhibitor, PCR Qualified Water, dNTPs and RNase H. Read about it in the Veriscript manual.
Interested to know more? click here for the product brochure

* The PCR process is covered by US Patents 4,683,195, and 4,683,202 and foreign equivalents owned by Hoffmann-La Roche AG.