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Stool Parasites

CE-IVD. For in vitro diagnostic use.

Amplidiag┬« Stool Parasites identifies the three most frequent enteric parasites rapidly from a single stool sample in a single reaction. This qualitative multiplex real-time PCR test is designed to be used as the primary screening method for stools incoming for parasite analysis. The current screening method, microscopy, is laborious, prone to errors and requires substantial expertise. Amplidiag Stool Parasites allows effective detection of Cryptosporidium spp., Giardia lamblia, Entamoeba histolytica and Dientamoeba fragilis. Microscopy can be targeted to samples which need further investigation, making the screening process more effective and accurate.


  • Sample material: DNA extracted from stool
  • Product format: Multiplex real-time PCR kit
  • Result analysis and reporting: Automated with Amplidiag Analyzer software

Target Panel

  • Cryptosporidium spp.
  • Giardia lamblia
  • Entamoeba histolytica
  • Dientamoeba fragilis

Key Benefits

  • Directly from DNA extracted from stool
  • Optimize stool parasite screening by detecting the most common pathogens by multiplex real-time PCR
  • Target microscopic analysis only to samples needing further investigation
  • All targets detected and reported from a single sample
  • Suited for high-volume screening
  • Automated results analysis with Amplidiag Analyzer software
  • Option for workflow automation with Amplidiag Easy

About stool parasites

Pathogenic protozoa are the main causative agents of parasite-associated gastroenteritis due to their ability to multiply in humans. Protozoa are microscopic, one-celled organisms that can be free-living or parasitic in natural reservoirs, and may be transmitted from human to human by fecal-oral route through contaminated food, water, and direct person-to-person contact. Protozoa are able to multiply in humans and they may cause serious infections starting from the transmission of a single organism. Four most common and medically important causative organisms are Entamoeba histolyticaGiardia lamblia, Cryptosporidium parvum and Dientamoeba fragilis, causing diarrhea and a wide variety of other prolonged symptoms, such as colitis, abdominal discomfort, and nausea.

These infections are treated primarily with specific antiprotozoal therapy. Traditional diagnostics, based on microscopy, is both laborious and challenging as there are multiple closely related non-pathogenic organisms. The sensitivity of microscopy depends also on the target organisms and prior medication. Enzyme immunoassay tests are capable of detecting only one or two targets. Overall, typically three separate samples are needed to reach sufficient sensitivity in diagnosing such infections, with substantial possibilities for improvement in diagnostic processes.