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Modified Oligos

 

Dye Modifications

            Modification
Dye Abs   Em Ext. Coeff. Mol. Weight 5' Int 3'
Alexa Fluor 350 346   442 19.000 409,35 x x x
Dy-415 418   467 34.000 552,00 x x x
ATTO 425 436   484 45.000 499,00 x x x
ATTO 465 453   508 75.000 393,00 x x x
Bodipy FL 504   510 70.000 391,30 x x x
Alexa Fluor 488 495   519 71.000 630,00 x x x
FAM 495   520 83.000 474,50 x

FITC 495   520 73.000 505,00 x x x
Fluorescein 495   520 83.000 504,00

x
Fluorescein-dT 494   522 75.000 472,71
x
ATTO 488 501   523 90.000 687,00 x x x
Oregon Green 488 496   524 76.000 510,00 x x x
Oregon Green 514 506   526 85.000 609,40 x x x
Rhodamine Green 503   528 74.000 472,00 x x x
TET 521   536 73.000 612,30 x

ATTO 520 516   538 110.000 464,00 x x x
JOE 520   548 73.000 603,40 x x x
Yakima Yellow 530   549 84.000 654,30 x

Bodipy 530/550 534   551 77.000 514,31 x x x
HEX 535   556 73.000 681,20 x

Alexa Fluor 555 555   565 150.000 940,00 x x x
Dy-549 553   566 150.000 916,00 x x x
Bodipy TMR-X 544   570 56.000 610,55 x x x
Cy3 552   570 150.000 444,60 x

ATTO 550 554   576 120.000 691,00 x x x
TAMRA 544   576 90.000 512,58/612,70 x x
Rhodamine Red 560   580 129.000 770,00 x x x
ATTO 565 563   592 120.000 608,00 x x x
ROX 575   602 82.000 632,78 x x x
Texas Red 583   603 116.000 818,00 x x x
Cy3.5 588   604 150.000 544,70 x

LightCycler 610 590   610 n.a. 761,48 x x x
ATTO 594 601   627 120.000 903,00 x x x
DY-480 XL 500   630 50.000 612,68 x x x
DY-610 610   630 80.000 743,00 x x x
ATTO 610 615   634 150.000 488,00 x x x
LightCycler 640 625   640 n.a. 824,68 x x x
Bodipy 630/650 625   640 101.000 662,60 x x x
ATTO 633 629   657 130.000 649,00 x x x
Alexa Fluor 647 650   665 239.000 957,00 x x x
Bodipy 650/665 650   665 101.000 645,60 x x x
ATTO 647N 644   669 150.000 743,00 x x x
Cy5 649   670 250.000 470,63 x

DY-649 655   674 250.000 942,00 x x x
LightCycler 670 645   678 215.000 470,63 x

Cy5.5 675   694 250.000 570,74 x

ATTO 680 680   700 125.000 623,00 x x x
LightCycler 705 685   705 215.000 570,74 x

DY-682 690   709 140.000 906,00 x x x
ATTO 700 700   719 120.000 663,00 x x x
ATTO 740 740   764 120.000 565,00 x x x
DY-782 782   800 170.000 932,00 x x x

Non Fluorescent Modifications

Modifications   PSF HPLC PAGE   5' 3'2 Int'
Amino-Modifier C6   x x x   x    
Amino-Modifier C12   x x x   x    
Aminolink C3/C6/C7   x x x     x  
Biotin 3'   x x x     x  
Biotin 5'   x x x   x    
Biotin-TEG   x x x   x x  
Digoxigenin     x     x x x
Phosphate (PHOs)   x x x   x x  
Thiol-Modifier C3     x       x  
Thiol-Modifier C6     x     x    

Base, Sugar & Backbone Modifications

Modifications   PSF HPLC PAGE   5' 3' Int
2'-Deoxyinosine (INO)   x x x     x x
2'-Deoxyuridine (URI)   x x x     x x
2',3' dideoxyA (5' ddA)   x x x       x
2',3' dideoxyG (5' ddG)   x x x       x
2',3' dideoxyC (5' ddC)   x x x       x
2',3' dideoxyT (5' ddT)   x x x       x
2',3' dideoxyC (3' ddC)   x x x     x  
5-Methyl-dC   x x x     x x
Amino C6-dT   x x x       x
Biotin-dT   x x x       x
dSpacer (deoxy abasic)   x x x       x
Spacer C3   x x x     x x
Wobble (defined ratio)   x x x       x