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X-CLARITY™ The ultimate solution for tissue clearing

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The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient. Examine your samples with a new perspective with the X-CLARITY™.

 

 

Features

Sectioning is time. We cut the time

Time is your most valuable asset in research. Imagine the time you spend preparing, sectioning, labeling, mounting, imaging, reconstructing, and analyzing hundreds upon thousands of ultra-thin slices. Now imagine never having to section again. Keep your samples whole with X-CLARITY™, your ultimate solution for electrophoretic tissue clearing.

Accelerate your research with the X-CLARITY™

Logos Biosystems licensed and independently developed the original CLARITY technology to make significant improvements for tissue clearing efficiency and reproducibility. With the world’s first commercial electrophoretic tissue clearing system, a new polymerization system, and ready-to-use reagents, tissue clearing has never been faster or simpler.

electrophoretic tissue clearing electrophoretic tissue clearing

WHAT IS CLARITY ?

CLARITY is a tissue clearing method that produces structurally sound and transparent whole tissues ready for multiple rounds of antibody labeling and imaging. The method has opened up a world of possibilities, from tracing neural circuitry to exploring the relationship between structure and function.
The main steps are as follows:

  1. Hydrogel Infusion

  2. A fixed tissue sample is incubated in a hydrogel monomer solution to allow hydrogel monomers to diffuse uniformly throughout the sample.
    The X-CLARITY™ Hydrogel Solution Kit is a ready-to-use acrylamide solution with a VA-044 thermal initiator that has been found to produce tissue samples that can be cleared rapidly and thoroughly.

  3. Hydrogel-Tissue Hybridization

  4. Once the hydrogel monomer solution has fully permeated the tissue sample, the monomers are polymerized to embed the tissue in a hydrogel matrix. The sample is heated, causing the thermal initiator to release free radicals and initiate radical polymerization of the hydrogel monomers, covalently linking the biomolecules in the tissue sample to a sturdy hydrogel network, preserving molecular information and structural integrity. The process is done in a strictly anaerobic environment, as oxygen is highly reactive with free radicals, making it a strong inhibitor of polymerization.
    The X-CLARITY™ Polymerization System is a standalone, automated system that eliminates the need for extra equipment such as a vacuum pump, desiccator, tanks of nitrogen gas, or a water bath. Samples can simply be placed in well plates or conical tubes to polymerize pre-treated samples in a heated and strictly anaerobic environment. Users control vacuum strength, temperature, and a timer through a simple touchscreen interface.

  5. Tissue Clearing

  6. Once the tissue-hydrogel hybrid has been formed, lipids are broken up through electrophoresis in the presence of ionic detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.
    The X-CLARITY™ Tissue Clearing System is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample.
    The X-CLARITY™ can clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Electrophoretic Tissue Clearing Solution is a premixed SDS-based buffer optimized for use with the X-CLARITY™ Tissue Clearing System.

  7. Labeling and Imaging

  8. The tissue-hydrogel hybrid is placed in an RI matching solution prior to imaging to optimize transparency.
    X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching medium.
    X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

  • How does the X-CLARITY™ work?

  • X-CLARITY™ Polymerization System

    The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify hydrogel-tissue hybridization, a crucial step for optimal tissue clearing. Proper hydrogel polymerization used to require multiple steps, which included degassing the sample in a desiccator chamber with a vacuum pump, replacing the oxygen with an inert gas, and then heating the sample in a water bath. The X-CLARITY™ Polymerization System reduces these steps into one. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface. A ready-to-use X-CLARITY™ Hydrogel Solution Kit simplifies the process even further.

    X-CLARITY™ Tissue Clearing System

    The X-CLARITY™ Tissue Clearing System is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. The X-CLARITY™ Tissue Clearing System can clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology.

    Complicated wires are combined into a single cable for power supply and control. Improved electrodes generate a uniform electric field that eliminates uneven voltages and currents. A timer allows precise control over the clearing time by automatically turning off the current at the end of the allotted time. An active cooling system minimizes the probability of artifacts arising due to poor temperature control. Buffer circulation ensures consistent buffering capacity, temperature control, and elimination of electrophoretic byproducts. This advanced system ensures that tissue clearing occurs efficiently and rapidly. Moreover, the X-CLARITY™ Tissue Clearing System successfully produces transparent tissues that are efficiently penetrated by and labeled with macromolecules such as antibodies or oligonucleotides. This allows for the 3D imaging of large tissues at single-cell resolution.

    Accessories and Reagents

    X-CLARITY™ Polymerization System

    Reagents

    Buy online:
    C1310X X-CLARITY Hydrogel Solution Kit includes X-CLARITY Hydrogel Solution (1 L) and X-CLARITY Polymerization Initiator (2.5 g) Log in to see the price Buy

    Buy online:
    C13104 X-CLARITY Polymerization Initiator, 2.5 g Log in to see the price Buy

    Accessories

    Buy online:
    C20002 X-CLARITY Heat Block for 6 x 50 mL tubes Log in to see the price Buy

    Buy online:
    C20003 X-CLARITY Heat Block for flat-bottom plates Log in to see the price Buy

    X-CLARITY™ Tissue Clearing System

    Reagents

    Buy online:
    C13001 Electrophoretic Tissue Clearing Solution, 12 X 1 L Log in to see the price Buy

    Buy online:
    C12001 Tissue Container (Whole Mouse Brain) - 20 units Log in to see the price Buy

    Buy online:
    C12004 Mouse Brain Slice Holder (Chamber Height: 4 mm) Log in to see the price Buy

    Buy online:
    C12002 Container Holder for 1 Tissue Container Log in to see the price Buy

    Buy online:
    C12007 Whole Rat Brain Holder Log in to see the price Buy

    The following holder sets accommodate samples of various shapes and sizes. The diverse spacers are sandwiched between mesh plates with 1.5 or 0.6 Φ perforations to keep even the smallest samples in place.


    7 1.5 Φ or 0.6 Φ Mesh Plates
    6 Spacers for 6 Slices
    3 silicone rings
    Product Sheet

    Buy online:
    C12010 1.5 diam. Holder for 36 Mouse Brain Slices Log in to see the price Buy


    7 1.5 Φ or 0.6 Φ Mesh Plates
    6 Spacers for 1 Slice
    3 silicone rings
    Product Sheet

    Buy online:
    C12011 1.5 diam. Holder for 6 Slices Log in to see the price Buy
    Buy online:
    C12021 0.6 diam. Holder for 6 Slices Log in to see the price Buy

    2 1.5 Φ or 0.6 Φ Mesh Plates
    1 Spacer for 1 Sample
    3 silicone rings
    Product Sheet

    Buy online:
    C12012 1.5 diam. Holder for 1 Sample Log in to see the price Buy
    Buy online:
    C12022 0.6 diam. Holder for 1 Sample Log in to see the price Buy


    2 1.5 Φ or 0.6 Φ Mesh Plates
    1 Spacer for 6 Mouse Brains
    3 silicone rings
    Product Sheet

    Buy online:
    C12013 1.5 diam. Holder for 6 Mouse Brains Log in to see the price Buy
    Buy online:
    C12023 0.6 diam. Holder for 6 Mouse Brains Log in to see the price Buy


    3 1.5 Φ or 0.6 Φ Mesh Plates
    2 Spacers for 24 Samples
    3 silicone rings
    Product Sheet

    Buy online:
    C12014 1.5 diam. Holder for 48 Samples Log in to see the price Buy
    Buy online:
    C12024 0.6 diam. Holder for 48 Samples Log in to see the price Buy


    3 1.5 Φ or 0.6 Φ Mesh Plates
    2 Spacers for 96 Samples
    3 silicone rings
    Product Sheet

    Buy online:
    C12015 1.5 diam. Holder for 192 Samples Log in to see the price Buy
    Buy online:
    C12025 0.6 diam. Holder for 192 Samples Log in to see the price Buy

    X-CLARITY™ Mounting Solution

    X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching medium. X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

    Reagents


    A water-soluble agent for refractive index homogenization of cleared tissues

    Buy online:
    C13101 X-CLARITY Mounting Solution (25 mL) Log in to see the price Buy


    Buy online:
    C13102 X-CLARITY Mounting Solution (Value Pack), 10 X 25 mL Log in to see the price Buy