Home > Plate reading > Fluorescence Polarization readers

Fluorescence Polarization readers

Fluorescence polarization detection is based on the excitation of a fluophore, similar as in standard Fluorescence Intensity (FI). However, the difference is that the optical system includes polarizing filters: the samples in the microplate are excited using polarized light and depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not. Large molecules such as proteins in solution rotate relatively slowly because of their size, and will emit polarized light when excited with polarized light. Conversely, fast rotation of smaller molecules will result in a depolarized signal. A low level of polarization indicates that fluorescently labelled  molecules move freely in the solution. A high level of polarization then means that a larger molecular complex is present, which rotates slowly due to the size. This technique allows the monitoring of increase in molecular volume due to receptor-ligand, DNA-protein, or peptide-protein binding or a decrease in molecular volume due to dissociation or enzymatic degradation.

Please select the reader of your interest:

^ProductGuide(Detection modes, Special);