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Countstar



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Countstar Automated Cell Counter

With the automatic cell counter Countstar, you can quickly and accurately determine

  • cell count
  • cell concentration
  • cell viability

through easy operations, reducing the complexity and time required by haemocytometers, while increasing precision.

The Countstar Automated Cell Counter is a cell counting and analyzing instrument developed on the basis of classic trypan blue staining. It integrates advanced optical imaging technology and intelligent image recognition technology.
The Countstar can complete accurate an cell counting in less than 20 seconds and provides:

  • cell concentration
  • cell viability
  • average diameter
  • average circularity
  • diameter range
  • aggregation of cells in histograms.

All the data are saved automatically and can be output in JPEG, EXCEL or PDF. In addition, the Countstar software system also provides cultivation time chart (CTC), overlay analysis and other statistical and analytical functions.

Easy and convenient: load the slide with 20 µl sample, click start and the measurement will be completed in 5 - 20 seconds.

 

Wide range compatibility: The Countstar can be used with cells having a diameter of 5 - 180 µm, such as blood cells, mammalian cells, insect cells, algae and some planktons.

 

Product performance: Measure cell properties in an all-round way

Accurate and clear results: 5-Megapixel color imaging and a wide sample collection range ensures clear and accurate visualization. The unique fixed-focus design guarantees cross comparisons and time-sequential comparisons.

Comprehensive parameter analysis: cell concentration, viability, diameter, circularity and aggregation rate. The cell growth curve and statistical charts are automatically generated.

Strong and sustainable management: The database supports the sustainable management of test data. The multi-user access controls can ensure the quality control and data security of industrial users to the maximum extent.

Environment-friendly and cost-efficient consumables: The one-piece multi-slot design minimizes the wasting of resources and cuts down your test costs. The consumables are reusable.

Accuracy and reliability guaranteed by multiple technologies

The balance achieved by Countstar between HD imaging and a large sampling range in combination with intelligent image recognition and calibration technology contributes to a clear presentation of measurements.
Unique cell and particle measurement modes, a flexible and convenient parameter debugging and correction optimizing system and other features ensure high statistical consistency between the measurements of Countstar and those obtained through manual counting, and greatly reduce errors in parallel measurement with the latter.
For these reasons, Countstar Cell is the most trusted upgrade and substitute for manual counting.

Based on trypan blue staining, Countstar Cell has a measurement range of 1×104-3×107 cells/mL, with the optimal measurement range being 1×105-5×106 cells/mL. In addition to good similarity between its measurements and those of a haemocytometer (see Fig. 1 and Fig. 2), Countstar can avoid errors caused by manual counting, thus making it much more reliable than manual counting (see Fig. 3, Fig. 4 and Fig. 5).

Fig. 1: Seen from the result, no statistical difference is shown between Countstar and hemocytometer (Y = 0.9319x; R2= 0.9987) Fig. 2: Seen from the result, no statistical difference is shown between Countstar and hemocytometer(Y = 0.9771x; R2= 0.9985)

 

Fig. 3: CHO concentration as an example, no statistical difference is shown between Countstar and hemocytometer and the results of Countstar Cell are more stable. Fig. 4: CHO viability as an example, no statistical difference is shown between Countstar and hemocytometer and the results of Countstar Cell are more stable. Fig. 5: The concentration of CHO, MCF-7, HepG2, MDCK and Vero as an example, no statistical difference is shown between Countstar and hemocytometer and the results of Countstar Cell are more stable.

LUNA-II Automated Cell Counter

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Not available in Benelux
  • Fully automated cell counting and cell viability analysis
  • Autofocus technology for fast and reliable cell counting
  • Various histograms and cell-size gating
  • Unmatched counting accuracy and speed
  • Built-in computer and printer
  • The most affordable running cost currently on the market

New Autofocusing Technology

The LUNA-II™ automated cell counter has integrated a novel focusing mechanism based on liquid lens technology. The liquid lens does not use any mechanical moving parts to change the Z stage. Instead, the Z position of the sample is rapidly obtained by the application of a small voltage to the liquid lens. The elimination of moving parts removes noise and significantly reduces the need for servicing. Even after multi-million cycles of focusing, the liquid lens does not require repair or service. In combination with this novel focusing mechanism, the LUNA-II™ has integrated a new autofocusing algorithm optimized for cell counting.

liquid lens autofocusing mechanism

Reduced Counting Costs, Increased Convenience

COST EFFICIENT: The new LUNA™ Reusable Slide has been designed to work with the LUNA™ family of cell counters for cost-efficient and accurate cell counting. The LUNA™ Reusable Slide has the affordability of manual cell counting without the associated subjectivity and time.

CONVENIENT: LUNA™ Cell Counting Slides offer the ultimate counting experience. With no mess or cleanup, these disposable precision slides maintain the highest standard of cell counting accuracy.

Cell Counting Accuracy : De-Clustering

The LUNA-II™ automated cell counting algorithm has been recognized to have the best-in-class cell counting accuracy. The LUNA™ software has exceptional accuracy in cell de-clustering and can successfully count clumpy cells. The LUNA-II™ automated cell counter has inherited this well-known performance aspect of the LUNA™ software. Clumpy cells are de-clustered quickly and automatically, then counted as individual cells.

Erythrosin B And Trypan Blue-Compatible

Although the LUNA-II™ can detect and count unstained cells, viability information can only be obtained from stained cell solutions. The traditional trypan blue can be used with the LUNA-II™, as well as the safer Erythrosin B. Both dyes produce consistently accurate cell count and viability data with the LUNA-II™.

Read the following article for more information on Erythrosin B-stained cell viability data: “Application of a non-hazardous vital dye for cell counting with automated cell counters”.

Built – In Printer

A thermal printer is integrated in the LUNA-II™ automated cell counter, and the counting results can be printed immediately for record keeping purposes. Because the printer is already integrated in the LUNA-II™ automated cell counter, an additional cable connection is not required.

low-cost cell counting slides and highly accurate counting algorithm

Interactive Software Interface

Options for staining methods

The LUNA-II™ automated cell counter can count samples with or without trypan blue dye. The selection of trypan blue presence or absence can easily be chosen in the settings menu.

Cell size histogram and cell size based gating

After counting, cells can be gated based on their size information provided by the LUNA-II™. The specific size of cell populations Can easily be included or excluded on histograms.

Cell cluster map

Counted cells can be sorted with cell cluster map, into single cells, doublets, or triplets. The cell cluster map can be used to monitor changes in culture conditions or cell isolation/preparation protocols.

Review and Re-analysis option

The LUNA-II™ automated cell counter provides a powerful review option.

A separate PC unnecessary. Users can open cell images directly on the LUNA-II™ automated cell counter to check previous results.

Various file save options

The LUNA-II™ can store up to 1,000 counts onboard. After counting, the result is automatically saved in the memory, and previous count results can be exported via USB as a .CSV file for further analysis. The results and image data can be saved in three different file formats such as TIF, annotated TIF, or a PDF report file. The PDF report file contains all the data generated during/after the cell counting, i.e., the protocol used, cell counting results, date, raw/analyzed cell image(s), and histograms are included in the PDF report file.

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L40001 LUNA-II Automated Cell Counter (with built-in printer) Log in to see the price Buy
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L40002 LUNA-II Automated Cell Counter (without printer) Log in to see the price Buy
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L12008 LUNA Reusable Slide Log in to see the price Buy
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L12001 Cell Counting Slides, 50 Slides (100 Counts) Log in to see the price Buy
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L12002 Cell Counting Slides, 500 Slides (1000 Counts), 10 boxes Log in to see the price Buy
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L12003 Cell Counting Slides, 1000 Slides (2000 Counts), 20 boxes Log in to see the price Buy
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T13101 Log in to see the price Buy
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L13002 LUNA Biosafe Stain (200 tests), 2 X 1.0 ml Log in to see the price Buy
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B13101 LUNA Standard Bead (Bright Field), Concentration: 1.0 X 10e6/ml, Size: 10 um, 2 X 1.0 ml Log in to see the price Buy
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P12002 LUNA-II Printer Paper - thermal, 700 prints, 2 x 5 rolls Log in to see the price Buy
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U10005 USB Drive 16GB Log in to see the price Buy

LUNA-FL Dual Fluorescence Cell Analyzer

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  • Most affordable counting cost
  • Dual fluorescence optics for sensitive cell analysis
  • Cell counting with bright field optics
  • Unmatched cell counting accuracy
  • Optimized for primary cells, stem cells, PBMCs, splenocytes, etc.
  • Cell size gating
  • Interactive graphic user interface

Dual Fluorescence Cell Counting

The LUNA-FL™ Dual Fluorescence Cell Counter from Logos Biosystems is a quantum leap forward for automated cell counting and cell viability analysis. The LUNA-FL™ Dual Fluorescence Cell Counter gives you sensitive and accurate live/dead counts without cell type restrictions. Unlike immortalized cell lines, primary cells such as PBMCs, splenocytes, neutrophils, and stem cells have been difficult to count with conventional cell counters such as the Coulter counter or automated image-based cell counters utilizing bright field optics. Primary cells are often contaminated with undesirable debris that can be miscounted as cells with conventional cell counters. The LUNA-FL™ is equipped with dual fluorescence optics to overcome this problem. Live and dead cells are stained with green and red fluorescence dye, respectively, and subsequent cell images are analyzed with accurate image analyzing software (to read a general review of fluorescence cell counting, click here).

Basic overview of fluorescence cell counting mechanism and comparative data

Erythrosin B And Trypan Blue-Compatible

Although the LUNA-FL™ can detect and count unstained cells, viability information can only be obtained from stained cell solutions. The traditional trypan blue can be used with the LUNA-FL™, as well as the safer Erythrosin B. Both dyes produce consistently accurate cell count and viability data with the LUNA-FL™.

Read the following article for more information on Erythrosin B-stained cell viability data: “Application of a non-hazardous vital dye for cell counting with automated cell counters”.

Reduced Counting Costs, Increased Convenience

Cost Efficient: The new LUNA™ Reusable Slide has been designed to work with the LUNA™ family of cell counters for cost-efficient and accurate cell counting. The LUNA™ Reusable Slide has the affordability of manual cell counting without the associated subjectivity and time.

Convenient: The LUNA-FL™ is compatible with PhotonSlides™ and LUNA™ Cell Counting Slides. These disposable precision slides offer the ultimate counting experience with no mess or cleanup, while maintaining the highest standard of cell counting accuracy.

Gfp Transfection Efficiency Assay

With the LUNA-FL™ Dual Fluorescence Cell Counter, GFP expression analysis takes less than 30 seconds. Bright field and fluorescence cell images are analyzed to detect GFP-positive and negative cells, and a corresponding fluorescence intensity histogram is displayed for further analysis. The LUNA-FL™ Dual Fluorescence Cell Counter is integrated with on-board flow cytometry type gating software. Be confident in the accuracy of your results by adjusting the threshold of fluorescence intensity.

Automated GFP Transfection Analysis

Interactive Software Interface

Powerful on-board analysis

Once the LUNA-FL™ has counted your cells, you can check to see which cells were judged to be live or dead on the touch screen. Green and red circles indicate live and dead cells, respectively.

Cell size gating

Cells can be gated based on cell size. you can easily exclude or include the particles by simply dragging the ends of the histogram that graphically shows live and dead cell populations.

Image overlay

The analyzed images from each channel (bright field, green, and red) can easily be merged directly on the screen. The brightness of each color can be adjusted for accurate monitoring. These images can be saved to an external USB drive for your records and further analysis.

Data report

The analyzed data can be saved as a CSV file or a more detailed PDF report. Counting results, cell image data, and a various histograms are combined into a single PDF file and saved to an external USB drive.

Powerful, on-board cell count analysis and reporting

Compact And Fast Label Printer

LUNA-FL™ provides a compact mobile printer for further record keeping purpose. Thermal roll paper or label paper can be selected. This plug & play mobile printer is ready-to-use after the USB to USB connection.

compact mobile printer

Ordering Information

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L20001 LUNA-FL Automated Fluorescence Cell Counter Log in to see the price Buy
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L12008 LUNA Reusable Slide Log in to see the price Buy
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L12005 PhotonSlide, 50 Slides (100 Counts) Log in to see the price Buy
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L12006 PhotonSlide, 500 Slides (1000 Counts), 10 boxes Log in to see the price Buy
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L12007 PhotonSlide, 1000 Slides (2000 Counts), 20 boxes Log in to see the price Buy
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L12001 Cell Counting Slides, 50 Slides (100 Counts) Log in to see the price Buy
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L12002 Cell Counting Slides, 500 Slides (1000 Counts), 10 boxes Log in to see the price Buy
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L12003 Cell Counting Slides, 1000 Slides (2000 Counts), 20 boxes Log in to see the price Buy
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T13001 Trypan Blue Stain 0.4% (200 tests), 2 X 1.0 ml Log in to see the price Buy
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L13002 LUNA Biosafe Stain (200 tests), 2 X 1.0 ml Log in to see the price Buy
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F23001 AO/PI Cell Viability Kit (500 tests), 2 X 0.5 ml Log in to see the price Buy
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F23002 AO Cell Viability Kit (500 tests), 2 X 0.5 ml Log in to see the price Buy
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F23202 Yeast Viability Kit 1 (500 tests) Log in to see the price Buy
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F23004 PI stain for yeast (500 tests) 2 x 0,5 ml Log in to see the price Buy
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F23211 FDA (Fluorescein Diacetate) Staining Solution (500 tests), 2 X 0.5 ml Log in to see the price Buy
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F23212 Cell Dilution Buffer (500 tests), 5 x 20 ml Log in to see the price Buy
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F23213 Fluorescence Signal Enhancer 1 (500 tests), 2 X 0.5 ml Log in to see the price Buy
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F23102 LUNA-FL Calibration Bead (50 tests), 1 x 0.5 ml Log in to see the price Buy
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B13101 LUNA Standard Bead (Bright Field), Concentration: 1.0 X 10e6/ml, Size: 10 um, 2 X 1.0 ml Log in to see the price Buy
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P10001 LUNA Printer Log in to see the price Buy
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P10002 Log in to see the price Buy
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P13001 LUNA Printer Cleaning Pen Log in to see the price Buy
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U10005 USB Drive 16GB Log in to see the price Buy