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Microbial ATP Kit



Productnr.: 266-112

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266-112
Microbial ATP Kit HS: kit, 375 tests
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The Microbial ATP Kit HS is intended for determination of intracellular ATP in bacterial cells. It is used when you have bacterial samples that are contaminated by extracellular ATP as well as mammalian cells. The kit contains ATP Eliminating Reagent and Cell Lysis Buffer for rapid and easy degradation of the non-bacterial ATP.

The HS reagent used has a high sensitivity, making it possible to detect down to 5 bacterial cells ( depending on the sensitivity of the luminometer).  The high sensitivity is due to the high luciferase activity in the reagent, which has the extra advantage that if the reagent is contaminated during reconstitution the ATP background will be degraded by one order of magnitude every 38 min. After mixing the sample and ATP Reagent HS the light will decay with approx. 6%/min. This is slow enough to allow manual mixing, if a luminometer with dispensers is not available.

Please note that the number of bacterial cells and CFU is not the same, since one CFU might consist of several bacterial cells.  If the detection limit is not adequate it is possible to concentrate the sample by microfiltration. This will remove almost all extracellular ATP.

If you are in doubt that 266-112 Microbial ATP Kit HS is suitable for your assay, please don´t hesitate to contact us.

Measurement of ATP in microbial cells after the elimination of non-microbial ATP.

  • Fast assay: Results within minutes
  • Highly sensitive: 10-17 mol ATP (~ 5 bacteria)
  • Reliable: ATP standard in liquid form
  • Flexible: Choice of µ-plate or cuvette method

Specifications

Use: Rapid microbiology
special: Samples with mammalian cells and with extracellular ATP