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ATP Biomass kit HS



Productnr.: 266-311

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ATP Biomass Kit HS: kit, 375 tests Does not include swabs nor cuvettes
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The ATP Biomass Kit HS is intended for determination of the total ( both extracellular and intracellular) amount of ATP in a sample.  It is suitable for virtually all types of samples.

The HS reagent used has a high sensitivity, making it possible to detect down to 5 bacterial cells ( depending on the sensitivity of the luminometer).  The high sensitivity is due to the high luciferase activity in the reagent, which has the extra advantage that if the reagent is contaminated during reconstitution the ATP background will be degraded by one order of magnitude every 38 min. After mixing the sample and ATP Reagent HS the light will decay with approx. 6%/min. This is slow enough to allow manual mixing, if a luminometer with dispensers is not available.

Please note that the number of bacterial cells and CFU is not the same, since one CFU might consist of several bacterial cells.  If the detection limit is not adequate it is possible to concentrate the sample by microfiltration. This will remove almost all extracellular ATP.

Measurement of total ATP (extra- plus intra-cellular) in virtually all types of samples

  • Fast Assay: Results within minutes
  • Highly Sensitive: 10-17 mol ATP (~ 5 bacteria)
  • Reliable: Stable, Ready-to-use ATP Standard
  • Cost-efficient: No Standard curve required
  • Efficient Extractant: Animal and Microbial cells

Specifications

Use: Rapid microbiology
Special: Samples without mammalian cells and without extracellular ATP