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Specific Applications Oligos

 

PCR Oligos

Specifications for PCR Oligos

  • Optimized for standard PCR, RT-PCR and DNA sequencing
  • Synthesis average yield: ~ 40nmol (8 OD)
  • Primer lengths: 15-25 bases
  • Wobbles (non defined ratio) possible
  • Delivery: lyophilized in tube format
  • Fixed price per primer
  • Quality control by:
    • Trityl monitoring
    • OD measurement
    • MALDI-TOF MS

Cloning Oligos

Specifications for Cloning Oligos

Are you looking for the perfect oligo for your cloning applications?

  • DNA cloning, PCR cloning,
  • Gene synthesis, gene cloning and
  • Random gene library (incl. point mutations)

Experiments and results in increased numbers of correct clones in respect to mutations, deletions or substitutions in comparison to other oligo qualities.

  • Gene synthesis quality
  • For oligonucleotides 15-60 bases in length
  • High synthesis yields of 15-30 OD
  • 5’ Phosphate modification available
  • Wobbles (non defined ratio) possible
  • Quality control by OD measurement and MALDI-TOF MS
  • Delivery: lyophilized in tube format
  • Fixed price per oligonucleotide
  • Possibility to order:
    • Short oligos: 15-35 mer
    • Long Oligos: 36-60 mer

qPCR Probes

Dual Labeled Probes

Dual Labeled Probes contain a 5’ fluorescent reporter and a 3’ quencher. These fluorescent probes are used in quantitative / real time PCR

Molecular Beacons

Molecular Beacons are comparable with Dual Labeled Probes. They also contain a fluorescent reporter and a quencher at their 5' and 3' ends, respectively. In contrast to Dual Labeled Probes, the sequence of these probes is designed to allow the formation of a hairpin structure in which the fluorescent dye and the quencher are in close proximity

Available fluorescent Dyes and Quenchers

All fluorescent dyes and quenchers can be freely combined according to your needs.

5' Reporter   Abs [nm] Em [nm] Alternative to
FAM   495 520 AF 488
ATTO 488   501 523 Cy2, ORG 488, AF 488
TET   521 536
ATTO 520   516 538 CAL Fluor Gold 540
JOE   520 548
Yakima Yellow   530 549 VIC
HEX   535 556 VIC
DY-549   553 566 Cy3, AF 555 (NED)
Cy3   552 570
ATTO 550   554 576 NED
TAMRA   544 576
ATTO 565   563 592 PET
ROX   575 602 Texas Red, CAL Fluor Red 610 , AF 568
Texas Red   583 603 ROX, CAL Fluor Red 610, AF 568
Cy3.5   588 604 CAL Fluor Red 610, AF 568
LC 610   590 610
DY-610   610 630 ROX, Texas Red, AF 610
ATTO 610   615 634 ROX, Texas Red, AF 610
ATTO 633   629 657 LIZ
LC 640   625 640
ATTO 647N   644 669 Cy5, AF 647
Cy5   649 670 AF 647, Quasar 670
LC 670   546 678 Cy5, AF 647, Quasar 670
DY-649   655 674 Cy5, AF 647
Cy5.5   675 694 Quasar 705, AF 680
ATTO 680   680 700 AF 680
LC 705   685 705 Cy5.5, Quasar 705, AF 680

 

3' Quencher Quenching Range Max. Abs [nm]
Dabcyl 400 - 550 nm 453
Eclipse 390 - 625 nm 522
TAMRA 520 - 600 nm 544
BHQ1 480 - 580 nm 534
BHQ2 520 - 650 nm 579
BBQ650 550 - 750 nm 650

Specifications of Dual Labeled Probes and Molecular Beacons

  • Guaranteed minimum yields
  • HPLC purification is included
  • Delivery: lyophilized in tube format
  • Quality control by
    • - Trityl monitoring
    • - OD measurement
    • - MALDI-TOF MS
  • Price per probe
Synthesis Scale [µmol] 0.01 0.05 0.20 1.00
Yield [nmol] 15 25 40 75
Yield [OD] 3 5 8 15
Min. Yield [OD] 1 1,5 3 6
Length Restriction 5-40 5-40 5-40 5-40

A ready-to-use qPCR probe dilution buffer (10mM TrisCl; 1mM EDTA; pH 8) is provided along with all Dual Labeled Probes (1ml for up to 3 probes).

RAPD Primers

RAPD 10mer Kits contain 10mer primers specifically developed for Random Amplified Polymorphic DNA (RAPD) analysis, a technique used for DNA fingerprinting and genetic mapping.

In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown. Genomic DNA from different individuals gives different PCR products, allowing the identification of DNA polymorphisms that can be used to identify different individuals or as genetic markers.

The number of PCR products generated from each genomic DNA sample depends on the primer sequence, the genomic DNA sequence, and the genome size. Assuming that the priming sites of a 10mer RAPD primer are randomly distributed throughout a genome, the theoretical number of PCR products is approximately 2.5 x 10-9 x G, where G is the size of the haploid genome in base pairs. Using this calculation, a haploid genome of 2 x 109 bp, for example, is expected to give about 5 PCR products. This prediction is in close agreement with experimental results.

RAPD 10mers can be ordered either as complete kits or as single primers.

Specifications for RAPD Primers:

  • Synthesis yields up to 30nmol (3 OD)
  • Primer length: 10 bases
  • Available as single primers or complete kits
  • Delivery format: lyophilized
  • Quality control by
    • OD measurement
    • MALDI-TOF MS
  • Price per primer resp. per kit